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KMID : 0387720110220020144
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Korean Journal of Blood Transfusion
2011 Volume.22 No. 2 p.144 ~ p.150
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Evaluation of the Automated QWALYS-3 System for ABO and RhD Grouping and Unexpected Antibody Screening
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Ko Young-Hyun
Kim Ji-Myung
Koo Sun-Hoe
Lim Jin-Sook
Park Young-Choon
Kwon Kye-Chul
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Abstract
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Background: One hundred years after the discovery of the ABO blood group system by Landsteiner, agglutination is still the most widely used method for determining the blood grouping and for antibody detection. Yet the demand for suitable automated systems is obvious in the field of transfusion. QWALYS-3 (DIAGAST, Loos Cedex, France) is a fully automated system for determining the ABO and RhD grouping and for antibody screening. We evaluated the ABO and RhD grouping and antibody screening test of the QWALYS-3.
Methods: The ABO and RhD grouping and the antibody screening test were performed by the QWALYS-3 system and the results were compared to the manual methods for the ABO and RhD grouping and to the micro column agglutination method (DiaMed-ID system) for antibody screening.
Results: For 574 of 574 clinical samples, there was complete concordance between the QWALYS-3 and the manual methods for determining the ABO and RhD grouping. The concordance rate between the QWALYS-3 and the micro column agglutination method for antibody screening in 210 clinical samples was 97.1%.
Conclusion:The QWALYS-3 system had a good concordance rate compared to the manual method and the micro column agglutination method. The QWALYS-3 system was robust and it gave accurate results during this evaluation. The QWALYS-3 system will contribute to achieving full automation of all the necessary blood bank tests. (Korean J Blood Transfus 2011;22:144-150)
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KEYWORD
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QWALYS-3, ABO and RhD grouping, Antibody screening, Automation
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